Resistant Starch Assay Kit (K-RSTAR)

Is it possible to check if the resistant starch values for my samples are correct when analysed using the Resistant Starch Assay Kit (K-RSTAR)?
We highly recommend analysing the resistant starch control provided with the kit with each set of determinations. If the value obtained for the control is...
Mon, 18 Apr, 2016 at 12:44 PM
What type of the resistant starch is provided as a control with the Resistant Starch Assay Kit (K-RSTAR)?
The control provided with the Resistant Starch Assay Kit is Hylon VII, native high amylose maize starch (not retrograded). Product Page (K-RSTAR)
Mon, 18 Apr, 2016 at 12:45 PM
What is the reproducibility of the Resistant Starch Assay Kit (K-RSTAR)?
Standard errors of ± 5% are achieved routinely within our laboratory for samples containing > 2% of resistant starch. Higher errors are obtained for sa...
Mon, 18 Apr, 2016 at 12:45 PM
What is the lower limit of detection of the Resistant Starch Assay Kit (K-RSTAR)? What is the linear range of the glucose measurement?
The glucose measurement (incubation with GOPOD Reagent) is linear between 4 and 100 micrograms of glucose per assay. We recommend that the smallest differ...
Mon, 18 Apr, 2016 at 12:45 PM
Is it possible to know if I have to use the method for samples containing > 10 % RS content or the method for samples containing < 10 % RS content when analysing RS content using the Resistant Starch Assay Kit (K-RSTAR)?
This will depend on the type of sample being analysed.  Some information about RS content can be found in the scientific literature. If in doubt, you co...
Mon, 18 Apr, 2016 at 12:46 PM
Is it possible to use a different set up than shaking water bath set in linear motion as recommended in the Resistant Starch Assay Kit (K-RSTAR) for incubation of samples with pancreatic alpha-amylase/amyloglucosidase at 37°C?
You can use the air heated shaker. Sample and enzymes suspension needs to be continually suspended and shaken. We do not recommend using stirrer bars as ...
Mon, 18 Apr, 2016 at 12:46 PM
Is it possible to analyse wet samples (e.g. cooked pasta, cooked rice, soaked seeds) with the Resistant Starch Assay Kit (K-RSTAR)?
We recommend freeze-drying and milling the samples to pass a 1.0 mm sieve prior to the actual analysis. Freeze drying is recommended as resistant starch w...
Mon, 18 Apr, 2016 at 12:47 PM
Is it possible to analyse samples containing fat (e.g. sponge cake) with the Resistant Starch Assay Kit (K-RSTAR)?
We recommend de-fatting the sample prior to the actual analysis as high fat content may affect the performance of the kit. Samples can be defatted with pe...
Mon, 18 Apr, 2016 at 12:47 PM
How can we verify that the resistant starch has been dissolved completely in KOH after enzyme digestion in the Resistant Starch Assay Kit (K-RSTAR)?
The resistant starch will dissolve readily in the cold KOH. It is well known that more reliable results are obtained if the dissolution in KOH is done in ...
Mon, 18 Apr, 2016 at 12:47 PM
Is it necessary to add sodium azide to the sodium maleate buffer used in the Resistant Starch Assay Kit (K-RSTAR)?
The sodium azide is a preservative preventing the microbial growth in the buffer. It can be excluded from the buffer without affecting the performance of ...
Mon, 18 Apr, 2016 at 12:48 PM