One unit is the amount of enzyme required to release one micromole of glucose from starch per minute, under standard assay conditions (starch substrate) (10 mg/mL starch; pH 4.5; 40˚C).
Or
One unit is the amount of enzyme required to release one micromole of p-nitrophenol from p-nitrophenol beta-maltoside (in the presence of excess beta-glucosidase) (10 mM pNP betamaltoside; pH 4.5; 40˚C).


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