Amyloglucosidase (Aspergillus niger) (E-AMGDF)

Why does the Amyloglucosidase Assay Reagent (R-AMGR3) contain PNP-beta-D-maltoside and not PNP-alpha-D-maltoside, since amyloglucosidase is specific for alpha-1,4-D-linked glucans?
Amyloglucosidase acts reasonably rapidly on maltose linked to PNP, but not on glucose linked to PNP (alpha-linked). Therefore, we have prepared a reagent...
Wed, 24 Feb, 2016 at 3:37 PM
What is the definition of units for Amyloglucosidase (E-AMGDF)?
  One unit is the amount of enzyme...
Mon, 21 Mar, 2016 at 3:59 PM
In what absorbance range should I use Amyloglucosidase (E-AMGDF) with Amyloglucosidase Assay Reagent (R-AMGR3)?
The Amyloglucosidase Assay Reagent (R-AMGR3) is optimised for use in absorbance range 0.2-0.9. Dilute enzyme to get within this range. Product Page
Wed, 24 Feb, 2016 at 3:42 PM
Can Amyloglucosidase Assay Reagent (R-AMGR3) be used to measure amyloglucosidase (E-AMGDF) in the presence of α-glucosidase?
No, the substrate is acted on by both of these enzymes and significant amounts of α-glucosidase would interfere.  However, most amyloglucosidase preparati...
Mon, 21 Mar, 2016 at 4:00 PM
Can the Amyloglucosidase Reagent (R-AMGR3) be used to measure Amyloglucosidase (E-AMGDF) levels in malted barley and what is a suitable extraction procedure to use for ground malt?
The amyloglucosidase (AMG) present in ground malt is most probably of fungal origin, i.e. A. niger.  The flour should be extracted with 100 mM sodium acet...
Mon, 21 Mar, 2016 at 4:00 PM