Enzymatic hydrolysis as per K-GLUM, relies on specific enzymes that only target certain linkages. The enzyme mixture used in the kit is designed based on the simplified definition of the glucomannan structure, the actual glucomannan structure can be more complex and contain a small percentage of diverse or inaccessible linkages.
As a result, the enzyme mixture used in the kit may not fully hydrolyse the glucomannan, leaving some oligosaccharides and underestimating total sugar content compared to acid hydrolysis that is used to prepare samples for HPLC analysis.
Depending on the specific characteristics of the sample, some glucomannan materials may therefore yield lower results, which can be attributed to inherent variability in sample composition (e.g., exact structure of the polysaccharide), enzyme accessibility or general assay limitations.
These results are normal for enzymatic assays involving complex carbohydrates and do not necessarily reflect product purity or quality.
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