The glucose and mannose standard (bottle 8) is just a standard solution of these sugars, there is no glucomannan present so glucomannan calculation and hydrolysis is not required for this standard.
0.1 mL of the standard should be applied to the assay.
Please see as follows:
The glucose / mannose content in the standard should be confirmed as follows:
For glucose this equates to:
((2.84 x 180.16) / (6300 x 1 x sample volume)) X ΔA glucose
For mannose this equates to:
((2.86 x 180.16) / (6300 x 1 x sample volume)) X ΔA mannose