Amylazyme Tablets (T-AMZ)

Why is calcium added to the assay buffer that is used with the Amalyzyme tablets (T-AMZ)?
Alpha-Amylase requires calcium for stability. Product Page
Tue, 22 Mar, 2016 at 10:15 AM
Are Cibacron-blue amylose tablets the same as Amylazyme tablets (T-AMZ)?
No. Amylazyme tablets are similar in principle except that the tablet components hydrate rapidly and give more reproducible results. Cibacron blue amyl...
Tue, 22 Mar, 2016 at 10:16 AM
How do I measure alpha-amylase in malted wheat? Should I use Format 1 or 2 from the Amylazyme (T-AMZ) booklet?
Use Format 1. Product Page
Tue, 22 Mar, 2016 at 10:16 AM
In Amylazyme tablet (T-AMZ) assay procedure can centrifuging be used in the final clarification step rather than filtering? The results differ by 0.5 absorbance units.
It is necessary to filter because of the haze associated with the flour slurry. If you centrifuge all of the haze will not be removed and this gives the ...
Tue, 22 Mar, 2016 at 10:17 AM
How is the Amylazyme (T-AMZ) standard curve made?
Standard curves for Amylazyme method are prepared using pure alpha-amylase enzyme preparations which have been standardised against the Ceralpha method in...
Thu, 25 Feb, 2016 at 11:26 AM
Can the the Amylazyme (T-AMZ) method be used to measure alpha-amylase activity in the gut contents (duodenum, jejenum and ileum)?
We would suggest the Amylazyme (T-AMZ) method at a pH of 6.9. Product Page
Tue, 22 Mar, 2016 at 10:19 AM
Can you recommend a quick assay for residual alpha-amylase in whey protein concentrate powders?
The Amylazyme tablets (T-AMZ) will work best for your application (Format 2 in the kit booklet). Using format 2 you can get the greatest sensitivity. ...
Tue, 22 Mar, 2016 at 10:20 AM
What is the concentration of AZCL-Amylose in Amylazyme tablets (T-AMZ)?
Amylazyme tablets contain 30% w/w of AZCL-Amylose; the weight of the tablets is 100 mg (i.e. 30 mg of AZCL-Amylose per tablet). Product Page (T-AMZ...
Fri, 25 Mar, 2016 at 11:35 AM
I have tried Amylazyme tablets (T-AMZ) on baker’s flour but the assay is not detecting any activity. Why?
The level of Amylase in bakers flour is quite low. If the Amylazyme method is used, you should use Format 2; for greater sensitivity and increase the inc...
Tue, 22 Mar, 2016 at 10:20 AM
What is the correct concentration of Stopping Reagent for use in the Amylazyme (T-AMZ) assay?
For fungal and cereal alpha-amylases, use 10 mL or 2% tris base solution (pH ~ 8.5). For bacterial alpha-amylase it is best to use a solution of higher ...
Tue, 22 Mar, 2016 at 10:21 AM