How to prepare the required 78% ethanol solution that is used in the Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)? : Megazyme

How to prepare the required 78% ethanol solution that is used in the Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)?

Modified on: Fri, 22 Apr, 2016 at 2:23 PM

A volume reduction occurs on mixing water with 95% ethanol (or IMS). 1 L of 78% ethanol is prepared by adding 821 mL 95% ethanol (or IMS) to 207 mL H2O.
If using a 1 L volumetric flask, this is best accomplished by placing 821 mL 95% ethanol (or IMS) into the volumetric flask. Dilute to volume with deionised water. Mix well. Check the level and if necessary add more deionised water to bring it back up to the 1 L mark.

Product Page (K-RINTDF)

Vincent is the author of this solution article.

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