COMPARISON OF E-MAST 70306 AND 190222
APPEARANCE OF E-MAST LOT 70306 AND LOT 190222
The difference in appearance between the lots is due to the production process and purification from a crude preparation.
E-MAST Lot 190222 has a higher protein content and slightly higher glycerol concentration, so the higher viscosity of Lot 70306 is not due to these components. The higher viscosity in Lot 70306 may be due to a higher polysaccharide content (e.g. arabinoxylan) and this would have been more effectively removed in the process used to prepare Lot 190222a.
STABILITY OF E-MAST 190222a:
In the presence of 5 mM calcium chloride in diluted E-MAST Lot 190222a the a-amylase activity was much more stable with a loss of only ~ 4 % over the 4 days, a similar decrease to that observed in E-MAST Lot 70306. This compares to a 35 % decrease in a-amylase activity in E-MAST Lot 190222 in the absence of added calcium chloride.
RECOMMENDATIONS FOR USE OF E-MAST & E-MASTP:
- In the Datasheets for E-MAST and E-MASTP it is now recommended that the E-MAST is diluted using a solution of 0.5 % sodium chloride (NaCl) plus 5 mM calcium chloride (0.75 g CaCl2.2H2O/L), and that E-MASTP (powder) is dissolved and diluted in the same solution.
- Store the prepared sodium chloride/calcium chloride solution at 4oC for at least 1 hour so that it is cooled prior to performing the dilution of E-MAST
- E-MAST diluted in the sodium chloride/calcium chloride solution will be stable for ~ 4 days when stored at 4oC.
- The Datasheets for E-MAST and E-MASTP provide details on how to obtain activities of DP in-line with what is required using the SKALAR and Gallery equipment.