Phytic Acid Assay Kit (K-PHYT)

When using the Phytic Acid Assay Kit (K-PHYT) for quantitative analysis what level of accuracy and repeatability can be expected?
The Phytic Acid Assay Kit  is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expecte...
Tue, 1 Oct, 2019 at 10:08 AM
What is the detection limit of the Phytic Acid Assay Kit (K-PHYT)?
Under the conditions of the standard assay procedure the detection limit of the Phytic Acid Assay Kit  is ~ 11.29 mg phosphorous/100g of original sample (or...
Tue, 1 Oct, 2019 at 10:08 AM
What is the linearity of the Phytic Acid Assay Kit (K-PHYT)?
The Phytic Acid assay is linear over the range of 0.5 to 7.5 μg of phosphorous per assay. Under the conditions of the standard assay procedure this correspo...
Tue, 1 Oct, 2019 at 10:09 AM
What does the Phytic Acid Assay Kit (K-PHYT) measure?
Firstly the definition of measurement should be clearly defined prior to the analysis, e.g. is the analysis for total phosphorus (this includes enzymaticall...
Tue, 1 Oct, 2019 at 10:09 AM
What are the reference values for the sample supplied with the Phytic Acid Assay Kit (K-PHYT) and which methods have been used to certify them?
The certificate that Megazyme currently supplies for Phytic Acid Assay Kit  is the certificate of analysis for the kit (available where the product is locat...
Tue, 1 Oct, 2019 at 10:10 AM
Can liquid samples be tested for phytic acid using Phytic Acid Assay Kit (K-PHYT)?
Yes. Liquid samples can be tested for phytic acid using Phytic Acid Assay Kit . At Step 1 of the procedure (Sample Extraction), dilute a given volume of...
Tue, 1 Oct, 2019 at 10:10 AM
Can solid samples be tested for phytic acid using Phytic Acid Assay Kit (K-PHYT)?
Yes. Solid samples such as grain can be tested for phytic acid using Phytic Acid Assay Kit . For solid samples, perform step 1 of the Sample Extraction ...
Tue, 1 Oct, 2019 at 10:10 AM
How accurate is Phytic Acid Assay Kit (K-PHYT) for measuring unprocessed samples?
Unprocessed samples originating from grains usually contain ~ 97% of the total phosphorus content as phytic acid and therefore can be accurately measured us...
Tue, 1 Oct, 2019 at 10:11 AM
How accurate is Phytic Acid (Total Phosphorus) Assay Kit (K-PHYT) for measuring processed samples and does the protocol require any modifications?
When processed samples, which may contain various monophosphates not associated with phytic acid, are analysed the phytic acid content may be overestimate...
Mon, 18 Apr, 2016 at 11:36 AM
How is the sample dilution factor of 55.6 derived for the calculation used in the Phytic Acid Assay Kit?
The dilution factor of 55.6 is the overall dilution of the sample (after extraction) throughout the Standard Assay Procedure of K-PHYT (Part A step 2, and P...
Tue, 1 Oct, 2019 at 10:11 AM