The enzyme is supplied as an insoluble precipitate in ammonium sulphate so can be centrifuged to pellet the enzyme then re-suspended in an appropriate buffer used in the subsequent assay. Alternatively, the enzyme suspension can be mixed to become a uniform suspension immediately prior to aspirating then added directly to the assay buffer where it will readily become soluble assuming it is a high enough dilution e.g. 10-fold.
Modified on: Thu, 31 Aug, 2017 at 8:40 AM
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