We recommend adhering to the method as stated as this is how the kit has been validated.
Any deviation from this method will need to be internally validated.
However, if it is necessary to alter the procedure we would suggest running the control as the samples are run.
If the correct value for the control is obtained, it is possible to conclude the method deviation did not have any effect on the assay results.
We recommend observing samples closely to ensure they pellet correctly.
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