00:05 Introduction
06:46 Principle
06:46 Reagent Preparation
Procedure
09:45 Weighing of samples
10:13 Incubation with PAA and AMG
11:27 Preparation of Reagent Blanks
11:48 pH Adjustment & protease treatment
Method A – Measurement of TDF as HMWDF + SDFS
13:59 Alcohol Precipitation of HMWSDF
14:36 Filtration and separation of HMWDF (IDF + SDFP) and SDFS
17:21 Aa. Determination of HMWDF (IDF + SDFP)
18:31 Ab. Determination of SDFS
18:35 (i) Recovery, sub-sampling and concentration of SDFS
22:29 (ii) Chromatography of samples on HPLC
23:50 Calculations of TDF – as HMWDF (IDF + SDFP) plus SDFS
Method B – Measurement of TDF as IDF + SDFP + SDFS (allowing determination of IDF and SDFP + SDFS separately
27:06 Digestion of samples
27:50 Separation of IDF from SDFP & SDFS
29:47 Ba. Determination of IDF
31:15 Precipitation and recovery of SDFP
32:00 Bb. Determination of SDFP
32:21 Bc. Determination of SDFS
32:48 Calculations – TDF as IDF plus SDFP plus SDFS
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
Rapid Integrated Total Dietary Fiber Conference Paper (K-RINTDF) Video