The products are insoluble chromogenic substrates. They are used at a concentration of about 0.01 – 0.1% w/v in agar plate screening tests. These substrates can be autoclaved. The only AZCL-polymers that are less stable are AZCL-arabinan and AZCL-Galactan (which are not on your list). pH of medium should be in the range of 5-7.
If the activity you are screening for is produced by the organism you will get release of dye.
Prepare the required medium for the bacteria of interest and add to it the AZCL-substrates at a concentration of about 0.01 – 0.1% w/v. Then autoclave the media and pour the plates as normal.
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