00:03  Introduction
02:16  Principle


Method Pre-Requisites
03:34  Preparation of Fritted Crucibles
03:52  Sample Preparation
04:50  Reagent Preparation


Procedure
05:19  Weighing of Samples
05:44  Incubation with heat stable α-amylase
07:15  Incubation with Protease
08:11  Incubation with Amyloglucosidase


Measurement of "Prosky" Total Dietary Fiber as HMWDF
09:18  Precipitation of High Molecular Weight Soluble Dietary Fiber
09:59  Filtration & Washing of Dietary Fiber (HMWDF)
12:54  Determination of "Total" Dietary Fiber (HMWDF)
14:13  Calculations


Total Dietary Fiber Assay Kit (K-TDFR)