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00:03 Introduction
02:16 Principle


Method Pre-Requisites
03:34 Preparation of Fritted Crucibles
03:52 Sample Preparation
04:50 Reagent Preparation


Procedure
05:19 Weighing of Samples
05:44 Incubation with heat stable α-amylase
07:15 Incubation with Protease
08:11 Incubation with Amyloglucosidase


Measurement of "Prosky" Total Dietary Fiber as HMWDF
09:18 Precipitation of High Molecular Weight Soluble Dietary Fiber
09:59 Filtration & Washing of Dietary Fiber (HMWDF)
12:54 Determination of "Total" Dietary Fiber (HMWDF)
14:13 Calculations


Total Dietary Fiber Assay Kit