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Chapter 1: Kit Details
Chapter 2: Principle of the Assay
Chapter 3: Kit Contents
Chapter 4: Reagent Preparation
Chapter 5: Weighing of Samples, Addition of Buffer plus Amylase & AMG
Chapter 6: Addition of Tris Base and Inactivation of Amylase & AMG
Chapter 7: Incubation with Protease
Chapter 8A:   Determination of HMWDF & LMWSDF
               (i):  pH Adjustment & Addition of D-Sorbitol & Ethanol
              (ii):  Filtration & Recovery of HMWDF & LMWSDF
             (iii):  Ash & Protein Determination
             (iv):  Evaporation of Aqueous Ethanol & Recovery of LMWSDF
             (vi):  Desalting of Samples with Ion Exchange Resin
            (vii):  Liquid Chromatography of Desalted Samples
Chapter 8B:   Determination of IDF, HMWDF & LMWSDF
               (i):  pH Adjustment of the Incubation Mixture and Addition of D-Sorbitol
              (ii):  Filtration and Recovery of IDF, HMWDF & LMWSDF
Chapter 9: Calculation of Dietary Fiber Content

Integrated Total Dietary Fiber Assay Kit (K-INTDF)