Integrated Total Dietary Fiber Video Method with K-INTDF

00:00   Principle of the Assay
02:12   Kit Contents
02:40   Reagent Preparation
07:05   Weighing of Samples, Addition of Buffer plus Amylase & AMG
09:03   Addition of Tris Base and Inactivation of Amylase & AMG
10:12   Incubation with Protease
11:12   A. Determination of HMWDF & LMWSDF
11:16       (i): pH Adjustment & Addition of D-Sorbitol & Ethanol
13:20      (ii): Filtration & Recovery of HMWDF & LMWSDF
17:56     (iii): Ash Determination
18:13     (iv): Protein Determination
19:12      (v): Evaporation of Aqueous Ethanol & Recovery of LMWSDF
21:49     (vi): Desalting of Samples with Ion Exchange Resin
21:16    (vii): Liquid Chromatography of Desalted Samples
27:09   B. Determination of IDF, HMWDF & LMWSDF
29:07      (i): pH Adjustment of the Incubation Mixture and Addition of D-Sorbitol
31:10     (ii): Filtration and Recovery of IDF, HMWDF & LMWSDF
36:02   Calculation of Dietary Fiber Content

Integrated Total Dietary Fiber Assay Kit (K-INTDF)