00:00 Principle of the Assay
02:12 Kit Contents
02:40 Reagent Preparation
07:05 Weighing of Samples, Addition of Buffer plus Amylase & AMG
09:03 Addition of Tris Base and Inactivation of Amylase & AMG
10:12 Incubation with Protease
11:12 A. Determination of HMWDF & LMWSDF
11:16 (i): pH Adjustment & Addition of D-Sorbitol & Ethanol
13:20 (ii): Filtration & Recovery of HMWDF & LMWSDF
17:56 (iii): Ash Determination
18:13 (iv): Protein Determination
19:12 (v): Evaporation of Aqueous Ethanol & Recovery of LMWSDF
21:49 (vi): Desalting of Samples with Ion Exchange Resin
21:16 (vii): Liquid Chromatography of Desalted Samples
27:09 B. Determination of IDF, HMWDF & LMWSDF
29:07 (i): pH Adjustment of the Incubation Mixture and Addition of D-Sorbitol
31:10 (ii): Filtration and Recovery of IDF, HMWDF & LMWSDF
36:02 Calculation of Dietary Fiber Content
Integrated Total Dietary Fiber Assay Kit (K-INTDF)