The D-Mannitol assay will only measure the free, non-covalently linked monosaccharide. Oligosaccharides or polysaccharides can be measured after hydrolysis to free monosaccharides. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
Modified on: Tue, 1 Oct, 2019 at 10:20 AM
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