This is a method to prepare DL-isocitric acid from DL isocitric
acid-lactone but can also be used to convert DL isocitric acid-lactone to DL
isocitric acid in various samples.
To prepare 0.05 M DL isocitric acid: add 435 mg DL isocitric acid-lactone into 10 mL distilled water and pH to 9 using 1 M KOH. Heat in a boiling water bath for 10 min keeping the pH above 7. Cool and adjust to pH 7.4 with 2 M HCl and dilute to 25 mL.
Can D-Isocitric Acid Assay Kit (K-ISOC) be used to measure isocitric acid-lactone and if so what preparation method is used to convert it to isocitric acid?
Modified on: Fri, 24 Sep, 2021 at 12:40 PM
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