Some critical steps of the assay are as follows:
A2 should be read after approximately 10 min and you should ensure that the reaction has finished, i.e. measure the absorbance until it stops increasing. (Slight increases in absorbance of 0.001/min or less are acceptable).
The supernatants from both steps (1 and 2) of A. Sample Preparation should be clear.
What are the critical steps of the Lactulose Assay Kit (K-LACTUL)?
Modified on: Fri, 24 Sep, 2021 at 12:51 PM
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