In the low lactose assay kit (K-LOLAC), is it possible to filter samples or use a lower centrifugation speed?

Modified on Thu, 14 Oct, 2021 at 2:33 PM

We recommend that customers follow the instructions exactly as described in the assay procedure.

However it is possible to adapt this method to use filtration instead of centrifugation.

We recommend this as lower centrifugation speeds cause only a partial pellet to form.

We have found that the volumes in step A will need to be increased 5 fold to ensure enough filtrate is recovered for use in step B. Approx 3.0 mL will be recovered for use in step B

Step A	mL
Distilled water	4.5
Sample	2.5
Carrez I Solution	0.25
Carrez II Solution	0.25
Total Volume	7.5

2 mL of the clear filtrate is then used in step B.

Step B			mL
Distilled water			0.8
Clear Filtrate			2.0
Solution 1 (Buffer A)			0.2
Suspension 2 (GOX/Catalase)			0.4
hydrogen peroxide (30% w/w)			0.2
Total Volume			3.6