If the standard procedure is followed, the smallest differentiating recommended absorbance change (ΔA) is 0.04 (equivalent to ~ 40 μg of D-glucose/mL of sample). This is equivalent to ~ 2.7% of β-glucan in the sample.
The highest ΔA should be lower than the absorbance values obtained for 150 μg of glucose. This is equivalent to ~ 80% of β-glucan. If the expected β-glucan is higher, the sample should be diluted, 2-fold with water, prior to incubation with GOPOD Reagent.