You need a different standard curve. First, standardise the activity of the purified -cellulase on say CM-Cellulose 4M with Nelson/Somogyi reducing sugar method. Then, use this standardised enzyme to produce your standard curve. This is the way we produce our standard curves.
We use Cellazyme C tablets (T-CCZ) as a substrate for our cellulase assay but using different parameters than those outlined in the data booklet. How do I calculate the activity?
Modified on: Tue, 22 Mar, 2016 at 10:39 AM
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