You need a different standard curve. First, standardise the activity of the purified endo-cellulase on say CM-Cellulose 4M with Nelson/Somogyi reducing sugar method. Then, use this standardised enzyme to produce your standard curve. This is the way we produce our standard curves.
We use Cellazyme C tablets (T-CCZ) as a substrate for our cellulase assay but using different parameters than those outlined in the data booklet. How do I calculate the activity?
Modified on: Thu, 23 Sep, 2021 at 10:11 AM
Did you find it helpful? Yes NoSend feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.