You need a different standard curve. First, standardise the activity of the purified endo-cellulase on say CM-Cellulose 4M with Nelson/Somogyi reducing sugar method. Then, use this standardised enzyme to produce your standard curve. This is the way we produce our standard curves.
We use Cellazyme T tablets (T-CTZ) as a substrate for our cellulase assay but using different parameters than those outlined in the data booklet. How do I calculate the activity?
Modified on: Thu, 23 Sep, 2021 at 10:14 AM
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