You need a different standard curve. First, standardise the activity of the purified -cellulase on say CM-Cellulose 4M with Nelson/Somogyi reducing sugar method. Then, use this standardised enzyme to produce your standard curve. This is the way we produce our standard curves.
We use Cellazyme T tablets (T-CTZ) as a substrate for our cellulase assay but using different parameters than those outlined in the data booklet. How do I calculate the activity?
Modified on: Tue, 22 Mar, 2016 at 10:40 AM
Did you find it helpful?Send feedback
Sorry we couldn't be helpful. Help us improve this article with your feedback.