The Succinic Acid Assay Kit reaction is a negative reaction and therefore it starts with a high absorbance of ~ 1.5 and as the reaction proceeds the absorbance decreases therefore A2 will be expected to be lower than A1 for samples containing succinic acid. The more succinic acid present in the sample the more the absorbance will decrease. The change in absorbance (even though this is a negative change) between readings A1 and A2 corresponds to the amount of succinic acid present in the sample.
My A1 values for Succinic Acid Assay Kit (K-SUCC) blank and samples have an absorbance greater than 1.0. Is it correct for my A1 values to be this high?
Modified on: Mon, 18 Apr, 2016 at 3:24 PM
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