The AMG activity was determined with soluble starch as substrate (10 mg/mL) in 0.1 M sodium acetate buffer at pH 4.5 and 40˚C. One Unit is the amount of enzyme required to hydrolyse one micromole of maltose per minute (i.e. to release 2 micromoles of glucose). Glucose release is measured with Glucose Determination Reagent.
How was the activity determined for the amyloglucosidase (3260 U/mL on Soluble Starch) that is used in the Total Starch Assay Kit (AA/AMG) (K-TSTA)?
Modified on: Mon, 18 Apr, 2016 at 3:14 PM
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