How to calculate the"F factor" for the K-TSTA?

Modified on Wed, 1 Sep, 2021 at 2:21 PM

To calculate the F factor you use change in absorbance, the spectrophotometer should be blanked on the reagent blank and all absorbances should be read against thi at 510 nm.


F factor is calculated as follows:


100 μg of glucose (as per the supplied standard when using 0.1 mL) divided by the GOPOD absorbance value obtained for 100 μ g of glucose, read against the reagent blank at 510nm.


Please see below for an example calculation using an absorbance of 1.050.



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