Should CaCl2 be added before or after pH adjustment when making buffers?

Modified on Thu, 14 Oct, 2021 at 2:28 PM


We recommend that the CaCl2 is added after the pH adjustment of the solution.
The pH should also be checked after the addition to ensure it has not changed.

This is because CaCl2 is important for the activity of some enzymes, addition after pH adjustment will ensure it is not affected by the addition of strong acids or bases.

Was this article helpful?

That’s Great!

Thank you for your feedback

Sorry! We couldn't be helpful

Thank you for your feedback

Let us know how can we improve this article!

Select at least one of the reasons
CAPTCHA verification is required.

Feedback sent

We appreciate your effort and will try to fix the article