What is the maximum recommended temperature for avoiding starch retrogradation when drying samples with heat for use with the Total Starch Assay Kit (K-TSTA)?

Modified on Fri, 26 Nov, 2021 at 2:17 PM

We recommend freeze drying samples as heat can change the chemical structure of starch, for example dry heating is used to produce modified starches which are more resistant or unsusceptible to enzyme hydrolysis.  


If a dry heat is to be used we recommend not using a temperature higher than 40-50˚C in a forced air oven.


As Megazyme uses freeze drying for all of the samples tested this will need to be validated by the user for their lab.

We recommend drying a sample as above and comparing this to the same sample freeze dried.


Product Page (K-TSTA)

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