In this procedure total glucan is measured by hydrolysis with H2SO4, followed by incubation with exo-1,3-β-glucanase/β-glucosidase to ensure complete hydrolysis of laminari-oligosaccharides to glucose.
α-Glucan is measured specifically using a starch assay procedure. α-glucans found in maltodextrins, sucrose and trehalose are also included. β-glucan is then determined by difference.
Under the assay conditions (acid hydrolysis) beta-glucans present in the sample will be hydrolysed, including 1,3, 1,3:1,6, 1,3:1,4-β-glucan and most of 1,4-β-D-glucan (cellulose), which will lead to overestimation of 1,3:1,6-β-glucan.
K-EBHLG uses enzyme hydrolysis and is specific to the content of (1:3,1:6) beta glucan in your sample and cellulose will not be hydrolysed.
Product Page (K-YBGL)