Can the sample size used in the β-Glucan Assay Kit (Yeast & Mushroom) (K-YBGL) assay be reduced?

We recommend sticking to the procedure as stated as this is how the kit has been validated. 

However, we have reports from customers which have used this kit with 50 mg of sample with no reported issues. This would need to be validated internally by the customer but should not cause any issues as long as the concentration of the glucans are above the detection limit. 

Please note that by reducing the sample size the % error and reproducibility may be slightly affected.