Do I need to have two sets of glucose standards and reagent blanks for K-YBGL?

This depends on your assay batch.

If you analyze the glucose from both the total glucan and a-glucan procedures in a single batch of samples, then just one set of reagent blanks and one set of quadruplicate glucose standards will suffice. In the Megacalc calculator, enter the same values in both fields.

However, if you analyze the glucose from the total glucan procedure separately from the glucose derived from the a-glucan procedure, i.e., in two distinct batches, then you will require two sets of glucose standards and reagent blanks. One set for the total glucan test batch and another for the a-glucan test batch.

Megacalc will be filled with the relevant data for both.