Is it possible to remove free glucose and maltodextrins during the analysis of non resistant starch in the Resistant Starch (K-RSTAR) Assay Kit?

Modified on Tue, 9 Nov, 2021 at 9:34 AM

Please note that the measurement of non-resistant starch will also include any free glucose/maltodextrins present in the sample.

It is possible to wash the sample before the actual analysis with 80% ethanol, this step would remove free glucose and maltodextrins.

Please see the procedure below:

1. Add milled sample (~ 100 mg, weighed accurately) to a glass centrifuge tube (16 x 120 mm; 17 mL capacity).

2. Add 5.0 mL of aqueous ethanol (80% v/v), and incubate the tube at 80-85°C for 5 min. Mix the contents on a vortex stirrer and add another 5 mL of 80% v/v aqueous ethanol.

3. Centrifuge the tube for 10 min at 1,800 g (approx. 3,000 rpm) on a bench centrifuge. Discard the supernatant.

4. Resuspend the pellet in 10 mL of 80% v/v aqueous ethanol and stir on a vortex mixer.

5. Centrifuge as above and carefully pour off the supernatant.

6. Proceed as per step (a-ii) of K-RSTAR procedure (pg. 7 of the data booklet)