To measure alpha-amylase in wheat grains using the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) to test for the presence of alpha-amylase, do wheat grains have to be milled or can a blender be used?

Cereal grains do need to be milled to allow effective extraction of alpha-amylase (and other constituents). We recommend milling to pass a 0.5 mm screen. You may have some success with a cheap coffee grinder. However, the reproducibility will not be as good (it may be adequate for your requirements).

Product Page (K-CERA)