The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values may affect results, i.e. it may take longer to reach the end point; but the same end-point value should be obtained if not too far away from this pH value. Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment. Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Should the pH of the sample be adjusted when being analysed using the Glucose Determination Reagent (R-GLC4)? At what pH do glucose oxidase and peroxidase work?
Modified on: Wed, 10 Nov, 2021 at 3:26 PM
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