Is it possible to remove free glucose and maltodextrins during the analysis of non resistant starch in the Resistant Starch (K-RSTAR) Assay Kit?

Modified on Tue, 9 Nov, 2021 at 9:34 AM


Please note that the measurement of non-resistant starch will also include any free glucose/maltodextrins present in the sample. 


It is possible to wash the sample before the actual analysis with 80% ethanol, this step would remove free glucose and maltodextrins.


 Please see the procedure below:


1. Add milled sample (~ 100 mg, weighed accurately) to a glass centrifuge tube (16 x 120 mm; 17 mL capacity).


2. Add 5.0 mL of aqueous ethanol (80% v/v), and incubate the tube at 80-85°C for 5 min. Mix the contents on a vortex stirrer and add another 5 mL of 80% v/v aqueous ethanol.


3. Centrifuge the tube for 10 min at 1,800 g (approx. 3,000 rpm) on a bench centrifuge. Discard the supernatant.


4. Resuspend the pellet in 10 mL of 80% v/v aqueous ethanol and stir on a vortex mixer.


5. Centrifuge as above and carefully pour off the supernatant.


6. Proceed as per step (a-ii) of K-RSTAR procedure (pg. 7 of the data booklet)

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