When using the α-Amylase Assay Kit (Ceralpha Method) (K-CERA) and the absorbance is between 1.0–1.5, can the solution be diluted directly with an additional 3.0 mL of distilled water to reduce the absorbance by half before reading it?

Modified on Wed, 10 Nov, 2021 at 3:55 PM

If the absorbance value is above 1.2 then the assay will be limited by the amount of available substrate. Thus, you cannot simply dilute the colour.