In the β-Glucan Assay Kit (Yeast and Mushroom) (K-YBGL) procedure why was the hydrolysis with hydrochloric acid was replaced by hydrolysis with sulphuric acid?

Several acids and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H₂SO₄. For most samples, similar β-glucan values were obtained with both the optimized HCl and H₂SO₄ procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H₂SO₄ procedure resulted in significantly higher values.

(McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2)

The kit components have not been altered from previous lots.

Product Page (K-YBGL)